Antibody Usage In The Lab; Editor: Caponi, Laura; Editor: Migliorini, Paola; Spiral / comb / coil bound

Editor: Caponi, Laura; Editor: Migliorini, Paola
Springer Lab Manual; Spiral / comb / coil bound; 22 Figures, 5 Tables
154 pages
Published: June 1999
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
ISBN: 3540651489

This is a handy lab manual that allows quick and easy access to the techniques commonly used in analyzing antibody specificity. It describes some of the most useful immunological techniquesbased on antibodies, including ELISA, immunoblotting and immunoprecipitation protocols that provide useful tools for recognizing immunological specificities, together with basic immunofluorescence and immunohistochemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, combining the theoretical basis of each technique with sample protocols and a troubleshooting guide. The manual focuses on the various aspects of the protocols described to provide readers with the maximum possible information on each technique.Designed to develop practical skills and experience with techniques used to analyze antibody specificity.This text is a lab manual that permits quick and easy access to the techniques commonly used to analyze antibody specificity. Some of the most useful immunological techniques based on antibodies are described, including ELISA, immunoblotting and immunoprecipitation protocols.